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- Title
Simple in vitro migration assay for neural crest cells and the opposite effects of all-trans-retinoic acid on cephalic- and trunk-derived cells.
- Authors
Makoto Usami; Katsuyoshi Mitsunaga; Tomohiko Irie; Atsuko Miyajima; Osamu Doi
- Abstract
Here, we describe a simple in vitro neural crest cell (NCC) migration assay and the effects of all-transretinoic acid (RA) on NCCs. Neural tubes excised from the rhombencephalic or trunk region of day 10.5 rat embryos were cultured for 48 h to allow emigration and migration of NCCs. Migration of NCCs was measured as the change in the radius (radius ratio) calculated from the circular spread of NCCs between 24 and 48 h of culture. RA was added to the culture medium after 24 h at embryotoxic concentrations determined by rat whole embryo culture. RA (10-M) reduced the migration of cephalic NCCs, whereas it enhanced the migration of trunk NCCs, indicating that RA has opposite effects on these two types of NCCs.
- Publication
Congenital Anomalies, 2014, Vol 54, Issue 3, p184
- ISSN
0914-3505
- Publication type
Article
- DOI
10.1111/cga.12059