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- Title
Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt.
- Authors
AL-HERRAWY, Ahmad Z.; GAD, Mahmoud A.
- Abstract
Background: The aim of the present work was to investigate the prevalence and species of intestinal microsporidiosis among animals in Giza, Egypt. Methods: A total of 869 animal fecal samples were collected from domesticated animals (dogs, cats, rabbits, cattle, buffaloes, sheep, goats, donkeys and pigs) living in Giza, Egypt. Spores of microsporidia were concentrated from collected samples by centrifugation and finally stained with modified trichrome (MT) stain to detect microsporidial spores. Microsporidial spores in microscopically-positive samples were molecularly confirmed and identified using species-specific primers. Results: Spores of microsporidia were microscopically detected in 17.0% of the examined animal fecal samples. The highest and lowest rates of infection with intestinal microsporidia were recorded in dogs (33.3%) and buffaloes (6.9%), respectively. Molecularly, the obtained microsporidial spores were classified as Enterocytozoon bieneusi and E. intestinalis. Dual infection with both identified species was observed in fecal samples from buffalo, rabbit, goat, cat, pig and dog. Conclusion: Domestic animals may play a role in dissemination of intestinal microsporidiosis in the environment. Examined animals were infected with E. bieneusi in a higher percentage than E. intestinalis.
- Subjects
JIZAH (Egypt); MICROSPORIDIOSIS; DOMESTIC animal parasites; MICROSPORIDA; PROTOZOAN spores; DETECTION of animal droppings; POLYMERASE chain reaction; VETERINARY parasitology; DIAGNOSIS
- Publication
Iranian Journal of Parasitology, 2016, Vol 11, Issue 2, p195
- ISSN
1735-7020
- Publication type
Article