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- Title
The HPB-AML-I cell line possesses the propertiesof mesenchymal stem cells.
- Authors
Bambang Ardianto; Sugimoto, Takeshi; Kawano, Seiji; Kasagi, Shimpei; Jauharoh, Siti N. A.; Kurimoto, Chiyo; Tatsumi, Eiji; Morikawa, Keiko; Kumagai, Shunichi; Hayashi, Yoshitake
- Abstract
Background: In spite of its establishment from the peripheral blood of a case with acute myeloid leukemia (AML)- M1, HPB-AML-I shows plastic adherence with spindle-like morphology. In addition, lipid droplets can be induced in HPB-AML-I cells by methylisobutylxanthine, hydrocortisone, and indomethacin. These findings suggest that HPBAML- I is similar to mesenchymal stem cells (MSCs) or mesenchymal stromal cells rather than to hematopoietic cells. Methods: To examine this possibility, we characterized HPB-AML-I by performing cytochemical, cytogenetic, and phenotypic analyses, induction of differentiation toward mesenchymal lineage cells, and mixed lymphocyte culture analysis. Results: HPB-AML-I proved to be negative for myeloperoxidase, while surface antigen analysis disclosed that it was positive for MSC-related antigens, such as CD29, CD44, CD55, CD59, and CD73, but not for CD14, CD19, CD34, CD45, CD90, CD105, CD117, and HLA-DR. Karyotypic analysis showed the presence of complicated abnormalities, but no reciprocal translocations typically detected in AML cases. Following the induction of differentiation toward adipocytes, chondrocytes, and osteocytes, HPB-AML-I cells showed, in conjunction with extracellular matrix formation, lipid accumulation, proteoglycan synthesis, and alkaline phosphatase expression. Mixed lymphocyte culture demonstrated that CD3+ T-cell proliferation was suppressed in the presence of HPB-AML-I cells. Conclusions: We conclude that HPB-AML-I cells appear to be unique neoplastic cells, which may be derived from MSCs, but are not hematopoietic progenitor cells.
- Subjects
MEDICAL research; CELL culture; STEM cells; INDOMETHACIN; NONSTEROIDAL anti-inflammatory agents; CELL proliferation; EXTRACELLULAR matrix; CONNECTIVE tissues; CELL growth; BONE cells
- Publication
Journal of Experimental & Clinical Cancer Research (17569966), 2010, Vol 29, p163
- ISSN
1756-9966
- Publication type
Article
- DOI
10.1186/1756-9966-29-163