We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Characterization of single-domain antibodies against Foot and Mouth Disease Virus (FMDV) serotype O from a camelid and imaging of FMDV in baby hamster kidney-21 cells with single-domain antibody-quantum dots probes.
- Authors
Di Wang; Shunli Yang; Shuanghui Yin; Youjun Shang; Ping Du; Jianhong Guo; Jijun He; Jianping Cai Xiangtao Liu
- Abstract
Background: Foot-and-mouth disease (FMD) is a highly contagious disease that affects cloven-hoofed animals and causes significant economic losses to husbandry worldwide. The variable domain of heavy-chain antibodies (VHHs or single domain antibodies, sdAbs) are single-domain antigen-binding fragments derived from camelid heavy-chain antibodies. Results: In this work, two sdAbs against FMD virus (FMDV) serotype O were selected from a camelid phage display immune library and expressed in Escherichia coli. The serotype specificity and affinity of the sdAbs were identified through enzyme-linked immunosorbent assay and surface plasmon resonance assay. Moreover, the sdAbs were conjugated with quantum dots to constitute probes for imaging FMD virions. Results demonstrated that the two sdAbs were specific for serotype O and shared no cross-reactivity with serotypes A and Asia 1. The equilibrium dissociation constant (KD) values of the two sdAbs ranged from 6.23 nM to 8.24 nM, which indicated high affinity to FMDV antigens. Co-localization with the sdAb-AF488 and sdAb-QD probes indicated the same location of FMDV virions in baby hamster kidney-21 (BHK-21) cells. Conclusions: sdAb-QD probes are powerful tools to detect and image FMDV in BHK-21 cells.
- Subjects
FOOT &; mouth disease; COMMUNICABLE diseases; ANIMAL culture; SEROTYPES; ENZYME-linked immunosorbent assay; SURFACE plasmon resonance
- Publication
BMC Veterinary Research, 2015, Vol 11, Issue 1, p1
- ISSN
1746-6148
- Publication type
Article
- DOI
10.1186/s12917-015-0437-2