We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Multifunctionalized biocatalytic P22 nanoreactor for combinatory treatment of ER+ breast cancer.
- Authors
Chauhan, Kanchan; Hernandez-Meza, Juan M.; Rodríguez-Hernández, Ana G.; Juarez-Moreno, Karla; Sengar, Prakhar; Vazquez-Duhalt, Rafael
- Abstract
Background: Tamoxifen is the standard endocrine therapy for breast cancers, which require metabolic activation by cytochrome P450 enzymes (CYP). However, the lower and variable concentrations of CYP activity at the tumor remain major bottlenecks for the efficient treatment, causing severe side-effects. Combination nanotherapy has gained much recent attention for cancer treatment as it reduces the drug-associated toxicity without affecting the therapeutic response. Results: Here we show the modular design of P22 bacteriophage virus-like particles for nanoscale integration of virus-driven enzyme prodrug therapy and photodynamic therapy. These virus capsids carrying CYP activity at the core are decorated with photosensitizer and targeting moiety at the surface for effective combinatory treatment. The estradiol-functionalized nanoparticles are recognized and internalized into ER+ breast tumor cells increasing the intracellular CYP activity and showing the ability to produce reactive oxygen species (ROS) upon UV365 nm irradiation. The generated ROS in synergy with enzymatic activity drastically enhanced the tamoxifen sensitivity in vitro, strongly inhibiting tumor cells. Conclusions: This work clearly demonstrated that the targeted combinatory treatment using multifunctional biocatalytic P22 represents the effective nanotherapeutics for ER+ breast cancer.
- Subjects
BREAST cancer treatment; HORMONE therapy complications; CYTOCHROME P-450; REACTIVE oxygen species; PHOTODYNAMIC therapy; TAMOXIFEN
- Publication
Journal of Nanobiotechnology, 2018, Vol 16, p1
- ISSN
1477-3155
- Publication type
Article
- DOI
10.1186/s12951-018-0345-2