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- Title
Anti-inflammatory effects of a novel peptide designed to bind with NF-κB p50 subunit.
- Authors
WANG, Zhen-ping; CAI, Shao-xi; LIU, Dong-bo; XU, Xiang; LIANG, Hua-ping
- Abstract
Aim: To explore the anti-inflammatory effects of a novel peptide designed to bind with the NF-κB p50 subunit. Methods: The affinity of the peptide binding with p50 was measured with a biosensor. Levels of tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) from a human acute monocytic leukemia cell line (THP-1) treated with lipopolysaccharide (LPS) were measured using the ELISA method. In vivo anti-inflammatory effects of the peptide were tested with phorbol myristate acetate (PMA)-induced ear edema and zymosan A-induced peritonitis in mice. Results: The peptide has the ability to interact with the NF-κB p50 subunit and can effectively inhibit TNF-α and IL-6 production in the THP-1 cell line, PMA-induced ear edema and zymosan A-induced peritonitis in mice. Conclusion: The peptide may have therapeutic potential for the treatment of local acute inflammation.
- Subjects
PEPTIDES; INFLAMMATION treatment; TUMOR necrosis factors; INTERLEUKIN-6; CYTOKINES; PERITONITIS; APOPTOSIS
- Publication
Acta Pharmacologica Sinica, 2006, Vol 27, Issue 11, p1474
- ISSN
1671-4083
- Publication type
Article
- DOI
10.1111/j.1745-7254.2006.00442.x