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- Title
Exogenous addition of EGTA alters Ca<sup>2+</sup> and Fe<sup>2+</sup> content in broccoli hairy roots and affects sulforaphane production: based on transcriptomic analysis.
- Authors
Shi, Xiaotong; Bao, Jinyu; Ma, Shaoying; Zhao, Yi; Lan, Shimin; Cao, Jie; Li, Sheng
- Abstract
Glucoraphanin (GRA) produced by the hairy roots of broccoli (Brassica oleracea L. variety Italica Planch) is converted to the secondary metabolite Sulforaphane (SF), which has anticancer properties under specific conditions, and the synthesis of SF is significantly affected by both Ca2+ and Fe2+. Based on the screening of GRA, SF yields, and Epithiospecififier Protein (ESP) activity, the optimal concentration of exogenously added metal ion chelator Ethylene Glycol bis (2-aminoethyl) Tetraacetic Acid (EGTA) for the treatment of broccoli hairy roots and the optimal treatment time were 8.0 mM EGTA for 48 h. Fe2+ content in hairy roots of broccoli reached the lowest level at 48 h, and also significantly reduce the Ca2+ content, but the Ca2+ content recovered with the treatment time and reached the highest level at 48 h. Analysis of transcriptomics data indicated that the physiological and biochemical responses of broccoli hairy roots to exogenous additions of EGTA to metabolic pathways were mainly mediated by the regulation of the expression of relevant genes involved in signal transduction and amino acid metabolism. The expression of NSP2, NSP5, and ESP decreased with the increase of EGTA treatment time and was lowest at 48 h. The expression of 12 genes, such as AOP1.2.7, GSTF11 increased with the increase of EGTA treatment time, and the highest expression was found at 48 h. This study presented a theoretical basis for the study of the exogenous addition of EGTA to alter Ca2+ and Fe2+ contents and affect SF synthesis.
- Subjects
GENETIC regulation; AMINO acid metabolism; COLE crops; CELLULAR signal transduction; BROCCOLI; METAL ions
- Publication
In Vitro Cellular & Developmental Biology Plant, 2024, Vol 60, Issue 4, p456
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-024-10431-2