We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Identification of Novel Antileishmanial Chemotypes By High-Throughput Virtual and In Vitro Screening.
- Authors
Khan, Huma; Hakami, Mohammed Ageeli; Alamri, Mubarak A.; Alotaibi, Bader S.; Ullah, Nazif; Khan, Rasool; Khalid, Asaad; Abdalla, Ashraf N.; Wadood, Abdul
- Abstract
Background: Leishmaniasis is a deadly protozoan parasitic disease and a significant health problem in underdeveloped and developing countries. The global spread of the parasite, coupled with the emergence of drug resistance and severe side effects associated with existing treatments, has necessitated the identification of new and potential drugs. Objective: This study aimed to identify promising compounds for the treatment of leishmaniasis by targeting two essential enzymes of Leishmania donovani: trypanothione reductase (Try-R) and trypanothione synthetase (Try-S). Methods: High-throughput virtual and in vitro screening of in-house and commercial databases was conducted. A pharmacophore model with seven features was developed and validated using the Guner-Henery method. The pharmacophore-based virtual screening yielded 690 hits, which were further filtered through Lipinski's rule, ADMET analysis, and molecular docking against Try-R and Try-S. Molecular dynamics studies were performed on selected compounds, and in vitro experiments were conducted to evaluate their activity against the promastigote and amastigote forms of L. donovani. Results: The virtual screening and subsequent analysis identified 33 promising compounds. Molecular dynamics studies of two compounds (comp-1 and comp-2) demonstrated stable binding interactions with the target enzymes and high affinity. In vitro experiments revealed that 13 compounds exhibited moderate activity against both the promastigote (IC50, 41 µM–76 µM) and the amastigote (IC50, 44 µM–72 µM) forms of L. donovani. Compounds 1 and 2 showed the highest percent inhibition and the lowest IC50 values. Conclusion: The identified compounds demonstrated significant inhibitory activity against Leishmania donovani and stable interactions with target enzymes. These findings suggest that the compounds could serve as promising leads for developing new treatments for leishmaniasis.
- Subjects
PROTOZOAN diseases; PARASITIC diseases; LEISHMANIA donovani; LEISHMANIASIS; MOLECULAR dynamics
- Publication
Acta Parasitologica, 2024, Vol 69, Issue 3, p1439
- ISSN
1230-2821
- Publication type
Article
- DOI
10.1007/s11686-024-00899-8