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- Title
枸杞果实采后白霉病菌生物学特性及反式-2-己烯 醛对其防控研究.
- Authors
周月丽; 吴秀玲; 王 鹏; 张雯清; 孙 斌; 田 伟; 赵鲁迺克; 王军节
- Abstract
Objective To explore the biological characterization of the pathogen of white mold in Lycium barbarum L. fruits and to study on the prevention and control of the mold by trans-2-hexenal. Methods The Fusarium incarnatum isolated and identified from the white mold after Lycium barbarum L. fruits harvest was chosen as the research material. The dish assay method was used to study the biological characteristics of Fusarium incarnatum under varying nutrient substrates and culture conditions. The concentration of trans-2-hexenal was required as an effective treatment to the white mold of goji berries, which was determined by the method of the inhibit to the mycelial expansion in vitro, and the damage of inoculation by the pathogen, with the control of the disease by drug fumigation in vivo. Finally, the prevention effect of trans-2-hexenal on the disease was investigated through damage inoculation treatment after drug fumigation. Results The results showed that the ideal conditions for the mycelium growth of Fusarium incarnatum were potato dextrose agar (PDA) or potato sugar agar (PSA) medium, a temperature of 28℃, and total exposure to light. To achieve optimal spore production, the following conditions should be maintained: Use a PDA medium with a pH of 5, keep the temperature at 28℃, and ensure complete darkness. To promote spore germination, maintain a pH between 7 to 8, keep the temperature at 28℃, expose the sample to light, and subject it to a temperature of 51℃ for 10 minutes, which was lethal for hyphae. For the best growth about the sources of carbon and nitrogen, the pectin and yeast powder were the most suitable, respectively. Trans-2-hexenal, used in varying concentrations, which could inhibit the mycelium growth of Fusarium incarnatum. The concentration for 50% of the maximal effect (EC50) and minimum inhibitory concentration (MIC) for this pathogen were 0.0184 and 0.0322 μL/mL, respectively. Meanwhile, trans-2-hexenal was both control and prevent effects on the white mold of goji berries. The most effective concentration of trans-2-hexenal to fumigating was 40.0 μL/L. Conclusion It can be concluded that PDA or PSA medium, yeast powder as a nitrogen source, total light, and 28℃ temperature are enjoyed by the growth of the white mold pathogen in goji berries. This information can be used to choose an appropriate nitrogen fertilizer during production and a condition of low temperature and avoid light during storage. Additionally, trans-2-hexenal at 40.0 μL/L has been identified as a potential treatment to white mold disease of goji berry. These findings provide a foundation of effectively preventing, controlling the disease, and developing eco-friendly drugs.
- Publication
Journal of Food Safety & Quality, 2024, Vol 15, Issue 15, p102
- ISSN
2095-0381
- Publication type
Article
- DOI
10.19812/j.cnki.jfsq11-5956/ts.20240411001