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- Title
Icariin-Mediated Inhibition of NF-κB Activity Enhances the In Vitro and In Vivo Antitumour Effect of 5-Fluorouracil in Colorectal Cancer.
- Authors
Shi, De-Bing; Li, Xin-Xiang; Zheng, Hong-Tu; Li, Da-Wei; Cai, Guo-Xiang; Peng, Jun-Jie; Gu, Wei-Lie; Guan, Zu-Qing; Xu, Ye; Cai, San-Jun
- Abstract
Colorectal cancer (CRC) is an aggressive malignancy that has a poor prognosis. 5-Fluorouracil (5-FU) is a first line chemotherapeutic medication used in the treatment of gallbladder cancer; however, the efficacy is below satisfactory. Icariin is a natural compound that is conventionally reported to have activity against a variety of cancers. This study was carried out to investigate the anti-cancer effect of icariin in CRC cells and to determine whether the compound can enhance the antitumour activity of 5-FU. Cell proliferation and apoptosis were measured using an MTT assay and flow cytometry, respectively. The activity of transcription factor NF-κB was determined by EMSA method. The expression of apoptosis- and proliferation-related proteins was determined by western blotting. The in vivo antitumour effect of combination treatment with icariin and 5-FU on CRC was also assessed using a murine model of CRC. Icariin sensitized the CRC cells to 5-FU both in vitro and in vivo. The antitumour activity of icariin and its potentiating effect on the antitumour activity of 5-FU implicated the suppression of NF-κB activity and consequent down-regulation of the gene products regulated by NF-κB. Our results showed that icariin, suppressed tumour growth and enhanced the antitumour activity of 5-FU in CRC by inhibiting NF-κB activity. Therefore, we suggest that combination of icariin with 5-FU might offer a therapeutic benefit to the patients with CRC; however, further studies are required to ascertain this proposition.
- Subjects
COLON cancer treatment; ANTINEOPLASTIC agents; FLAVONOL glycosides; FLUOROURACIL; NF-kappa B; CANCER prognosis
- Publication
Cell Biochemistry & Biophysics, 2014, Vol 69, Issue 3, p523
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-014-9827-5