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- Title
Effect of Zymosan on Antioxidant and Immune Function of S Tumor-Bearing Mice.
- Authors
Xiao-li Liu; Ning Lin; Deng Zan; Jing-Jue Yuan; Dong-Lian Cai
- Abstract
The objective is to study the effect of zymosan on antioxidant and immune function of S tumor-bearing mice. Seventy Kunming mice were randomly divided into seven groups: a normal control group (NC), a tumor control group (TC), three dose groups of zymosan (low, medium, high), a cyclophosphamide (Cy) group, and a combination of zymosan and Cy group. The S tumor-bearing mice model was established by the inoculation of cancer cell suspension subcutaneously in the mouse's right anterior limb. At the 19th day, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activity in liver homogenate were analyzed. The reverse transcriptase-polymerase chain reaction was used to determine the mRNA expression levels of IL-2, TNF-α, and TGF-β. The activity of GSH-Px and SOD in the liver increased with the dose of zymosan, whereas the activity of MDA significantly decreased in the higher-dose groups of zymosan, compared to the TC group ( P < 0.01). In the zymosan groups, mRNA expression levels in tissues of S tumor-bearing mice were significantly higher for TNF-α and IL-2, but lower for TGFβ than in the Cy or TC group ( P < 0.01). The high-dose of zymosan markedly showed a depressant effect on S tumor, enhanced by the action of Cy that increased mRNA expression levels of TNF-α and IL-2. The mechanism of zymosan on the inhibition of tumor growth may be due to its ability to enhance the antioxidant and immune function in a dose-dependent manner.
- Subjects
ZYMOSAN; ANTIOXIDANTS; TUMORS; IMMUNITY; MALONDIALDEHYDE; GLUTATHIONE; SUPEROXIDE dismutase
- Publication
Cell Biochemistry & Biophysics, 2011, Vol 60, Issue 3, p225
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-010-9143-7