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- Title
Isolation and characterization of blackish-brown BY2-melanin accumulated in cultured tobacco BY-2 cells.
- Authors
Rafiqul Islam, Abul Taher Mohammed; Keita Shinzato; Hiroki Miyaoka; Kenji Komaguchi; Kanae Koike; Kenji Arakawa; Kenji Kitamura; Nobukazu Tanaka
- Abstract
The tobacco BY-2 cell line is one of the most utilized plant cell lines. After long-term culture, the cells turn brown to black, but the causal pigment is unknown. We successfully isolated a blackish-brown pigment from BY-2 cells cultured for 3 weeks. Morphological and spectroscopic analyses indicated that the pigment had similar features to a melanin-like substance reported previously. Furthermore, physicochemical analyses revealed that this pigment possessed most of the properties of melanin-like pigments. In addition, the high nitrogen content suggested that it differed from common plant melanins classified as allomelanins, suggesting a novel eumelanin-like pigment: "BY2-melanin". This is the first example showing that eumelanin-like pigments are produced in the cultures of plant cells for which the accumulation of melanin has not been reported. This tobacco BY-2 cell culture technique may represent a customizable and sustainable alternative to conventional melanin production platforms, with significant potential for industrial and pharmacological applications.
- Subjects
MELANINS; CELL culture; TOBACCO; PLANT cell culture; CELL lines; INDUSTRIAL capacity
- Publication
Bioscience, Biotechnology & Biochemistry, 2023, Vol 87, Issue 4, p395
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbac214