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- Title
Detection of 4 quinolone antibiotics by chemiluminescence based on a novel Nor-Biotin bifunctional ligand and SA-ALP technology.
- Authors
Han, Zhenyu; Sun, Tieqiang; Xu, Zehua; Fan, Longxing; Yun, Hanxuan; Ge, Xuejiao; Liu, Xiao; Liu, Ying; Ning, Bao'an
- Abstract
A simple and effective direct competitive chemiluminescence immunoassay for the detection of 4 kinds of quinolone antibiotics in milk was established using Nor-Biotin (biotin-modified norfloxacin [NOR]) bifunctional ligand and alkaline phosphatase–conjugated streptavidin signal amplification technology. The polyclonal antibody was obtained after the immunization of New Zealand White rabbits using norfloxacin-derived antigen. "Click chemistry" was used for the rapid and facile synthesis of the Nor-Biotin bifunctional ligand. After the optimization of the incubation time and reaction buffer, the direct competitive chemiluminescence assay method was developed and used for sensitive detection of 4 kinds of quinolone drugs (NOR, pefloxacin, ciprofloxacin, and danofloxacin). The IC50 of the 4 kinds of quinolone drugs ranged from 7.35 to 24.27 ng/mL, and the lowest detection limits ranged from 0.05 to 0.16 ng/mL, which were below their maximum residue levels, approved by the EU for treatment of food-producing animals. To demonstrate the applicability of the assay, artificially contaminated milk samples with the 4 quinolone drugs were analyzed. The mean recovery rates of the drugs ranged from 86.31% to 112.11%.
- Subjects
LIGANDS (Chemistry); CHEMILUMINESCENCE; CHEMILUMINESCENCE immunoassay; CHEMILUMINESCENCE assay; CLICK chemistry; STREPTAVIDIN
- Publication
Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 7, p1720
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbab081