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- Title
Characterization and comparison of recombinant honeybee chymotrypsin-like protease (HCLPase) expressed in Escherichia coli and insect cells.
- Authors
Matsuoka, Takuma; Kawashima, Takuji; Nakamura, Tadashi; Yabe, Tomio
- Abstract
We previously found a novel chymotrypsin-like protease in honeybee, designated as HCLPase. The recombinant enzyme expressed in insect cells was produced and compared to that inEscherichia coli. Both enzymes showed equivalent molecular size and specificity. However, HCLPase produced in insect cells showed higher specific activity. The C-terminal cleavage sites of HCLPase were phenylalanine, leucine, and tyrosine residues.
- Subjects
CHYMOTRYPSIN; ESCHERICHIA coli; BACTERIAL genetics; HONEYBEE genetics
- Publication
Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 7, p1401
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2017.1318698