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- Title
Metastasis-associated microRNA expression in canine uveal melanoma.
- Authors
Starkey, M. P.; Compston-Garnett, L.; Malho, P.; Dunn, K.; Dubielzig, R.
- Abstract
Background: Uveal melanoma (UM) is the most common primary intraocular tumour in dogs. There is no effective means of predicting whether a tumour will metastasize. microRNA (miRNA) metastasis signatures have been identified for several human cancers, including UM. Aims: In this study we investigated whether metastasizing and non-metastasizing canine UMs can be distinguished by miRNA expression levels. Materials and Methods: miRNA microarray profiling was used to compare miRNA expression in 8 metastasizing and 12 non-metastasizing formalin-fixed, paraffin-embedded (FFPE) primary UM biopsies. Results: Fourteen miRNAs exhibited statistically significant differences in expression between the metastasizing and non-metastasizing tumours. Class prediction analysis pinpointed 9 miRNAs which categorized tumours as metastasizing or non-metastasizing with an accuracy of 89%. Of the discriminating miRNAs, 8 were up-regulated in metastasizing UM, and included 3 miRNAs implicated as potential “metastasis activators” in human cutaneous melanoma. The expression of 4 of the miRNAs was subsequently measured using the quantitative reverse transcription polymerase chain reaction (RT-qPCR), and their up-regulation in metastasizing tumours validated. Conclusion: miRNA expression profiles may potentially be used to identify UMs that will metastasize, and miRNAs that are up-regulated in metastasizing tumours may be targets for therapeutic intervention.
- Subjects
MELANOMA; METASTASIS; MICRORNA; CANCER in dogs; BIOPSY
- Publication
Veterinary & Comparative Oncology, 2018, Vol 16, Issue 1, p81
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/vco.12315