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- Title
The Chinese Herbal Formula Ruyan Neixiao Cream Inhibits Angiogenesis of Precancerous Breast Lesions via Regulation of Ras/Raf/MEK/ERK Signaling Pathway.
- Authors
Lin, Shujun; Jiang, Xuefeng; Zhang, Guijuan; Xiao, Xinqin; Ma, Xinyi; Wu, Jieyan; Qiu, Dan; Li, Xinyuan; Yan, Xianxin; Ma, Min
- Abstract
Ruyan Neixiao Cream (RUc) is a traditional Chinese herbal formula which can effectively inhibit the angiogenesis of breast precancerous lesions. In order to reveal the specific mechanism, we carried out experiments in vitro and in vivo. We found that the conditioned medium of MCF-10AT cells treated with RUc transdermal solution (RUt) could significantly inhibit the proliferation, migration, invasion, tube formation of HUVECs and the capillary formation of rat aortic rings. RUt may down-regulate the expression of VEGF, MMP2, and MMP9 in MCF-10AT medium by down-regulating miR-21 and up-regulating TIMP-3 and RECK. We further confirmed in rats that the microvascular density of precancerous lesions decreased significantly after external use of RUc, which may be related to the inhibition of Ras/Raf/MEK/ERK signaling pathway related proteins. Presumptively, RUc may inhibit the angiogenesis of breast precancerous lesions by inhibiting Ras/Raf/MEK/ERK signaling pathway, thus relieving the inhibition of miR-21 on TIMP-3 and RECK, then down-regulating the secretion of angiogenic factors.
- Subjects
IN vitro studies; BREAST diseases; HERBAL medicine; NEOVASCULARIZATION inhibitors; IN vivo studies; ANIMAL experimentation; CELLULAR signal transduction; RATS; CELL motility; GENE expression; PATHOLOGIC neovascularization; TRANSFERASES; RESEARCH funding; CELL proliferation; OINTMENTS; PRECANCEROUS conditions; CHINESE medicine; PHARMACODYNAMICS
- Publication
Integrative Cancer Therapies, 2022, Vol 21, p1
- ISSN
1534-7354
- Publication type
Article
- DOI
10.1177/15347354211069397