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- Title
A Highly Sensitive and Selective Enzymatic Biosensor Based on Direct Electrochemistry of Hemoglobin at Zinc Oxide Nanoparticles Modified Activated Screen Printed Carbon Electrode.
- Authors
Palanisamy, Selvakumar; Karuppiah, Chelladurai; Chen, Shen‐Ming; Periakaruppan, Prakash
- Abstract
A sensitive enzymatic biosensor has been developed for the detection of hydrogen peroxide (H2O2), nitrite ( ${{\rm NO}{{- \hfill \atop 2\hfill}}}$) and trichloroacetic acid (TCA) by using hemoglobin (Hb) immobilized on activated screen printed carbon electrode (ASPCE) and zinc oxide (ZnO) composite. A pair of well defined redox peaks is observed with a heterogeneous electron transfer rate constant (Ks) of 5.27 s−1 for Hb at ASPCE/ZnO. The biosensor exhibits the detection of H2O2, TCA and ${{\rm NO}{{- \hfill \atop 2\hfill}}}$ in the concentration range of 0.5-129.5 µmol L−1, 2.5-72.5 mmol L−1 and 0.2-674 µmol L−1 with the detection limit of 0.083 µmol L−1, 0.12 mmol L−1 and 0.069 µmol L−1, respectively.
- Subjects
BIOSENSORS; ELECTROCHEMISTRY; HEMOGLOBINS; ZINC oxide; NANOPARTICLES
- Publication
Electroanalysis, 2014, Vol 26, Issue 9, p1984
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.201400304