We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Polymersomes Prepared from Thermoresponsive Fluorescent Protein-Polymer Bioconjugates: Capture of and Report on Drug and Protein Payloads.
- Authors
Wong, Chin Ken; Laos, Alistair J.; Soeriyadi, Alexander H.; Wiedenmann, Jörg; Curmi, Paul M. G.; Gooding, J. Justin; Marquis, Christopher P.; Stenzel, Martina H.; Thordarson, Pall
- Abstract
Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report where the components are in the polymersomes. To address these twin challenges, we synthesized the protein-polymer bioconjugate PNIPAM- b-amilFP497 composed of thermoresponsive poly( N-isopropylacrylamide) (PNIPAM) and a green-fluorescent protein variant (amilFP497). Above 37 °C, this bioconjugate forms polymersomes that can (co-)encapsulate the fluorescent drug doxorubicin and the fluorescent light-harvesting protein phycoerythrin 545 (PE545). Using fluorescence lifetime imaging microscopy and Förster resonance energy transfer (FLIM-FRET), we can distinguish the co-encapsulated PE545 protein inside the polymersome membrane while doxorubicin is found both in the polymersome core and membrane.
- Subjects
POLYMERSOMES; FLUORESCENT proteins; BIOCONJUGATES; CONJUGATED polymers; THERMAL analysis; FLUORESCENCE resonance energy transfer; ACRYLAMIDE derivatives
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 18, p5407
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201412406