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- Title
Molecular characterization of a retrotransposon in the Rhynchosciara americana genome and its association with telomere.
- Authors
Roberto Santelli
- Abstract
Abstract Non-LTR retrotransposons, also known as long interspersed nuclear elements (LINEs), are transposable elements that encode a reverse transcriptase and insert into genomic locations via RNA intermediates. The sequence analysis of a cDNA library constructed from mRNA of the salivary glands of R. americana showed the presence of putative class I elements. The cDNA clone with homology to a reverse transcriptase was the starting point for the present study. Genomic phage was isolated and sequenced and the molecular structure of the element was characterized as being a non-LTR retrotransposable element. Southern blot analysis indicated that this transposable element is represented by repeat sequences in the genome of R. americana. Chromosome tips were consistently positive when this element was used as probe in in-situ hybridization. Real-time RT-PCR showed that this retrotransposon is transcribed at different periods of larval development. Most interesting, the silencing of this retrotransposon in R. americana by RNA interference resulted in reduced transcript levels and in accelerated larval development.
- Subjects
TRANSPOSONS; GIANT chromosomes; EMBRYOLOGY; REVERSE transcriptase; TELOMERES; MESSENGER RNA; IN situ hybridization; ANIMAL genetics
- Publication
Chromosome Research, 2008, Vol 16, Issue 5, p729
- ISSN
0967-3849
- Publication type
Article