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- Title
rpoD gene expression as an indicator of bacterial pathogens in host plants.
- Authors
Narusaka, Mari; Shiraishi, Tomonori; Iwabuchi, Masaki; Narusaka, Yoshihiro
- Abstract
Pseudomonas, Ralstonia, and Xanthomonas are important genera of plant pathogenic bacteria that affect commercially cultivated crops. We developed an assay to assess the viability and development of bacterial pathogens in Arabidopsis thaliana by detecting rpoD mRNA using a quantitative reverse transcription-polymerase chain reaction (qRT-PCR). rpoD is a representative housekeeping gene that encodes the principal RNA polymerase sigma factor. Quantification of rpoD mRNA by qRT-PCR allowed easy detection of viability and development of the pathogens in infected plant tissue. Therefore, qRT-PCR technology provides new research opportunities for investigation of host-pathogen interactions.
- Subjects
PSEUDOMONAS; RALSTONIA; XANTHOMONAS; PLANT diseases; ARABIDOPSIS thaliana
- Publication
Journal of General Plant Pathology, 2011, Vol 77, Issue 2, p75
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-011-0298-x