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- Title
Development of a multiplex allele-specific primer PCR assay for simultaneous detection of QoI and CAA fungicide resistance alleles in Plasmopara viticola populations.
- Authors
Aoki, Yoshinao; Hada, Yosuke; Suzuki, Shunji
- Abstract
BACKGROUND: DNA-based diagnosis has become a common tool for the evaluation of fungicide resistance in obligate phytopathogenic fungus Plasmopara viticola. RESULTS: A multiplex allele-specific primer PCR assay has been developed for the rapid detection of fungicide resistance in P. viticola populations. With this assay, a glycine-to-alanine substitution at codon 143 of the P. viticola cytochrome b gene, which conferred QoI fungicide resistance, and a glycine-to-serine substitution at codon 1105 of the P. viticola cellulose synthase gene PvCesA3, which conferred CAA fungicide resistance, were detected simultaneously. CONCLUSION: It is suggested that the present assay is a reliable tool for the rapid and simultaneous detection of QoI and CAA fungicide resistance alleles in P. viticola populations. The assay required only 2 h from the sampling of symptoms to the detection of resistance alleles to both fungicides. Copyright © 2012 Society of Chemical Industry
- Subjects
PLASMOPARA viticola; FUNGICIDE resistance; GLYCINE; NUCLEIC acids; PESTICIDE resistance
- Publication
Pest Management Science, 2013, Vol 69, Issue 2, p268
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.3384