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- Title
Confirmation of Glucose Transporters through Targeted Mutagenesis and Transcriptional Analysis in Clostridium acetobutylicum.
- Authors
Zhang, Kundi; Jiang, Dandan; Liebl, Wolfgang; Wang, Maofeng; Gu, Lichuan; Liu, Ziyong; Ehrenreich, Armin
- Abstract
The solvent-producing bacterium Clostridium acetobutylicum is able to grow on a variety of carbohydrates. The main hexose transport system is the phosphoenolpyruvate-dependent phosphotransferase system (PTS). When the gene glcG that encodes the glucose transporter was inactivated, the resulting mutant glcG::int(1224) grew as well as the wild type, yet its glucose consumption was reduced by 17% in a batch fermentation. Transcriptomics analysis of the phosphate-limited continuous cultures showed that the cellobiose transporter GlcCE was highly up-regulated in the mutant glcG::int(1224). The glcCE mutation did not affect growth and even consumed slightly more glucose during solventogenesis growth compared to wild type, indicating that GlcG is the primary glucose-specific PTS. Poor growth of the double mutant glcG::int(1224)-glcCE::int(193) further revealed that GlcCE was the secondary glucose PTS and that there must be other PTSs capable of glucose uptake. The observations obtained in this study provided a promising foundation to understand glucose transport in C. acetobutylicum.
- Subjects
CLOSTRIDIUM acetobutylicum; MUTAGENESIS; CARBOHYDRATES; GLUCOSE; CELLOBIOSE; GLUCOSE transporters
- Publication
Fermentation (Basel), 2023, Vol 9, Issue 1, p64
- ISSN
2311-5637
- Publication type
Article
- DOI
10.3390/fermentation9010064