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- Title
高产阿魏酸酯酶菌株的筛选鉴定及产酶条件优化.
- Authors
王清龙; 陈园园; 刘延波; 刘洪亮; 王永华; 朱金晓; 张晓静; 潘春梅
- Abstract
In order to improve the content of ferulic acid in the brewing process of Baijiu, the ferulic acid esterase-producing strains were isolated and screened from strong-flavor (Nongxiangxing) Baijiu Daqu, and identified by morphological observation, physiological and biochemical tests and molecular biology techniques. Using ferulic acid esterase activity as evaluation index, the fermentation conditions were optimized by single factor experiments and response surface methodology. The results showed that a ferulic acid esterase-producing strain was obtained by screening, which was numbered as M2. It was identified as Bacillus cereus. The optimal fermentation conditions for the strain to produce ferulic acid esterase were inoculum 5%, fermentation temperature 30 ℃, initial pH 5.5, and time 72 h. Under the optimized conditions, the activity of ferulic acid esterase was 33.89 U/L, which was 10.03% higher than that before optimization, and it had a strong ability to produce ferulic acid esterase.
- Subjects
FERULIC acid; RESPONSE surfaces (Statistics); BACILLUS cereus; MOLECULAR biology; FERMENTATION; FLAVOR
- Publication
China Brewing, 2022, Vol 41, Issue 10, p83
- ISSN
0254-5071
- Publication type
Article
- DOI
10.11882/j.issn.0254-5071.2022.10.014