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- Title
野生菌核侧耳生物学特性及驯化栽培.
- Authors
王军芳; 宋国月; 高悦; 宋子坤; 马海霞
- Abstract
Pleurotus tuber-regium, generally used as medicine and edibles in China, has considerably high nutritional and medicinal values. A wild Pleurotus isolate collected from Limushan Natural Reserve of Hainan Tropical Rainforest National Park was purified and identified as P. tuber-regium by molecular analysis based on ITS sequences and morphological characteristics. The biological characteristics and domestic cultivation of the isolate were studied. The cross-hatch method was used to study the effects of different carbon and nitrogen sources, pH, and temperature on the mycelial growth rate under solid cultivation. The orthogonal tests were conducted on three optimal factors from four single factor experiments. The results showed that the optimal carbon and nitrogen sources for the mycelial growth of P. tuber-regium were sucrose and peptone respectively, under conditions of pH 7.0 at 35 °C. The spawn was the culture on corn grain substrate incubated at 25 °C under dark condition. The culture formula was 52% angiosperm wood chips, 25% corncob, 20% bran, 2% lime, and 1% gypsum. Primordium formed in 50 d of incubation and mature fruiting body in 55 days under the temperature of 28–32 °C, and humidity of 85%–90%. The present study is successful in domestic cultivation of P. tuber-regium, and supplies the primary data for future industrialized production of the fungus.
- Subjects
CHINA; FRUITING bodies (Fungi); WOOD chips; RAIN forests; EDIBLE fungi; PLEUROTUS
- Publication
Mycosystema, 2023, Vol 42, Issue 1, p395
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.220418