We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
基于Cfku70基因失活策略构建果生刺盘孢的高效 基因敲除底盘菌株.
- Authors
田欢焕; 杨文睿; 张兆辉; 姚立强; 张荣; 孙广宇; 梁晓飞
- Abstract
Colletotrichum fructicola is a devastating fungal phytopathogen that infects and damages many plants. In certain filamentous fungi, deleting ku70 or ku80, key genes of the nonhomologous end joining (NHEJ) pathway, can significantly boost homologous recombination and thereby increase target gene replacement frequency. In this study, Cfku70 and Cfku80 genes were identified from the C. fructicola genome, and the impacts of their inactivation on fungal biology and gene deletion efficiency were characterized. Deleting Cfku70 or Cfku80 did not affect fungal colony morphology, vegetative growth, sporulation, conidial germination, appressorium development and pathogenicity; in the meanwhile, Cfku70 gene deletion considerably improved deletion efficiencies of three tested genes. Our results demonstrate the usefulness of ΔCfku70 as a chassis strain enabling high-efficient target gene knock-out, and lay an important foundation for identifying novel virulence factors through the large scale gene deletion strategy in C. fructicola.
- Subjects
FUNGAL genes; DELETION mutation; FUNGAL morphology; GENE frequency; FUNGAL colonies
- Publication
Mycosystema, 2022, Vol 41, Issue 8, p1185
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.220179