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- Title
Interferon-γ and interleukin-4 production by human T cells recognizing Leishmania donovani antigens separated by SDS-PAGE.
- Authors
Bahrenscheer, JESPER; Kemp, MICHAEL; Kurtzhals, JØRGEN A. L.; Gachihi, GEORGE S.; Kharazmi, ARSALAN; Theander, THOR G.
- Abstract
Crude preparations of Leishmania donovani proteins were separated by preparative SDS-polyacryl-amide gel electrophoresis. Fractions of separated proteins were recovered by electroelution directly from the gel into separate chambers. The isolated protein fractions were tested for induction of proliferation and interferon-γ (IFN-γ) production in cultures of peripheral blood mononuclear cells (PBMC) from individuals who had recovered from visceral leishmaniasis caused by L. donovani. The release of interleukin-4 (IL-4) by PBMC stimulated with the isolated L. donovani antigen fractions was measured after treatment with phorbol-myristate-acetate and ionomycin. The cells proliferated in response to all protein fractions with molecular weights in the range <12 kDa to 85 kDa. In general, IFN-γ was secreted in response to stimulation with all the protein fractions, whereas IL-4 production was infrequently observed. The results show that T cells from individuals who have been cured of visceral leishmaniasis recognize and respond to a wide range of leishmanial antigens. There was no evidence of particular fractions constantly giving either IFN-γ- or IL-4-producing responses.
- Publication
APMIS, 1995, Vol 103, Issue 1-6, p131
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/j.1699-0463.1995.tb01088.x