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- Title
Der p2 recombinant bacille Calmette- Guerin priming of bone marrow-derived dendritic cells suppresses Der p2-induced T helper17 function in a mouse model of asthma.
- Authors
Qu, Shuo‐Yao; Ou‐yang, Hai‐Feng; He, Ya‐Long; Li, Zhi‐Kui; Shi, Jie‐Ran; Song, Li‐Qiang; Wu, Chang‐Gui
- Abstract
Background and objective Previous studies have demonstrated that our recombinant bacille Calmette- Guerin ( rBCG), which expresses Der p2 in house dust mite ( Der p2 rBCG) suppresses asthmatic airway inflammation by regulating the phenotype and function of dendritic cells ( DC) and reprogramming T helper ( Th) 0 cell differentiation into different T cell ( Th1/ Th2/ Treg) subtypes. However, the exact role of Der p2 rBCG in reprogramming Th17 differentiation and the relevant mechanisms are not known. The aim of this study was to examine whether Der p2 rBCG-mediated inhibition of allergic airway inflammation is mediated by regulating Th17 differentiation in a murine asthma model. Methods Primary mouse bone marrow-derived dendritic cells ( BMDC) were infected with Der p2 rBCG and adoptively transferred to Der p2-intranasally sensitized mice. The role of Der p2 rBCG- BMDC on the regulation of airway inflammation and Th17 cell differentiation was assessed. Results Adoptive transfer of Der p2 rBCG- BMDC suppressed airway inflammation and mucin secretion. Der p2 rBCG- BMDC inhibited excessive Th17 immune responses but not BCG- BMDC. Furthermore, Der p2 rBCG decreased jagged-2 and increased delta-like-4 expressions on BMDC to a greater extent than BCG. Conclusions These findings suggest that DC plays a key role in Der p2 rBCG-induced immunoregulation. Der p2 rBCG also displayed a potent inhibitory effect on Th17 differentiation, and these findings increase our understanding of the cellular basis of Der p2 BCG-mediated inhibition of asthma.
- Subjects
BCG vaccines; RADUMERIS tasmaniensis; BONE marrow; DENDRITIC cells; T helper cells; LABORATORY mice; ASTHMA
- Publication
Respirology, 2014, Vol 19, Issue 1, p122
- ISSN
1323-7799
- Publication type
Article
- DOI
10.1111/resp.12198