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- Title
Occurrence of all- cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3) in roughscale sole Clidoderma asperrimum flesh lipids.
- Authors
Fukuda, Yuuki; Ando, Yasuhiro
- Abstract
Fatty acid analysis of roughscale sole Clidoderma asperrimum flesh lipids was carried out by gas chromatography. An unidentified peak appeared in the chromatogram in the elution region of ≥C24 fatty acids. After enrichment by solvent partitioning, reversed-phase thin-layer chromatography (TLC), and argentation TLC, the peak component was subjected to structural analyses. The partially hydrogenated products after reaction with hydrazine hydrate gave seven isomers of cis-hexacosenoic acid (26:1). Gas chromatography-mass spectrometry (GC-MS) analyses of their dimethyl disulfide adducts identified the monounsaturates as 5-, 8-, 11-, 14-, 17-, 20-, and 23-26:1. The peak component was assigned to all- cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3). GC-MS analyses of the 4,4-dimethyloxazoline derivative and methyl ester confirmed this structure. This fatty acid is a rare, very long-chain polyunsaturated fatty acid (VLCPUFA). The concentrations of the acid found in roughscale sole were 0.69 ± 0.34% ( N = 5) of total fatty acids in flesh lipids. Roughscale sole appears to be characterized by the occurrence of 26:7n-3, which is lacking in popular sources of methylene-interrupted VLCPUFA, such as vertebrate retina, spermatozoa, and herring.
- Subjects
FATTY acids; LIPIDS; GAS chromatography; CHROMATOGRAMS; THIN layer chromatography; ISOMERS; MASS spectrometry
- Publication
Fisheries Science, 2011, Vol 77, Issue 5, p875
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1007/s12562-011-0387-y