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- Title
TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration.
- Authors
Yang, Huilin; Cao, Cheng; Wu, Chunshen; Yuan, Chenxi; Gu, Qiaoli; Shi, Qing; Zou, Jun
- Abstract
Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion. This increase inhibited IκB phosphorylation and NF-κB activation, detected by western blot analysis. Meanwhile, in a rabbit model, MRI analysis revealed significant recovery of signal intensity in the degenerative discs of rabbits receiving cells transplantation, than receiving cells treated with a TGF-β1 inhibitor or saline. These findings indicated that enhanced TGF-β1 production recovered the degeneration of intervertebral disc. And also immunohistochemical staining detected enhanced collagen II expression in the rabbits treated with cell transplantation. However, the NF-κB positive cells were significantly less than other two control groups. Thus, cell therapy promoted TGF-β1 expression in nucleus pulposus, leading to anti-inflammatory effects via the inhibition of NF-κB, and the amelioration of disc degradation due to increased expression of collagen II and aggrecan in degenerative intervertebral disc.
- Subjects
IMMUNOSTAINING; CELLULAR therapy; DEGENERATION (Pathology); TRANSFORMING growth factors; MAGNETIC resonance imaging; INTERVERTEBRAL disk diseases
- Publication
Scientific Reports, 2015, p13254
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep13254