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- Title
Creating Anti-icing Surfaces via the Direct Immobilization of Antifreeze Proteins on Aluminum.
- Authors
Gwak, Yunho; Park, Ji-in; Kim, Minjae; Jin, EonSeon; Kim, Hong Suk; Kwon, Myong Jong; Oh, Seung Jin; Kim, Young-Pil
- Abstract
Cryoprotectants such as antifreeze proteins (AFPs) and sugar molecules may provide a solution for icing problems. These anti-icing substances protect cells and tissues from freezing by inhibiting ice formation. In this study, we developed a method for coating an industrial metal material (aluminum, Al) with AFP from the Antarctic marine diatom, Chaetoceros neogracile (Cn-AFP), to prevent or delay ice formation. To coat Al with Cn-AFP, we used an Al-binding peptide (ABP) as a conjugator and fused it with Cn-AFP. The ABP bound well to the Al and did not considerably change the functional properties of AFP. Cn-AFP-coated Al (Cn-AFP-Al) showed a sufficiently low supercooling point. Additional trehalose coating of Cn-AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity. This metal surface-coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.
- Subjects
CRYOPROTECTIVE agents; ANTIFREEZE proteins; ICING (Meteorology) -- Physiological effect; ALUMINUM; THERAPEUTIC immobilization
- Publication
Scientific Reports, 2015, p12019
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep12019