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- Title
Neutrophil Adhesion and the Release of the Free Amino Acid Hydroxylysine.
- Authors
Galkina, Svetlana I.; Fedorova, Natalia V.; Ksenofontov, Alexander L.; Serebryakova, Marina V.; Golenkina, Ekaterina A.; Stadnichuk, Vladimir I.; Baratova, Ludmila A.; Sud'ina, Galina F.; Müller-Taubenberger, Annette
- Abstract
During infection or certain metabolic disorders, neutrophils can escape from blood vessels, invade and attach to other tissues. The invasion and adhesion of neutrophils is accompanied and maintained by their own secretion. We have previously found that adhesion of neutrophils to fibronectin dramatically and selectively stimulates the release of the free amino acid hydroxylysine. The role of hydroxylysine and lysyl hydroxylase in neutrophil adhesion has not been studied, nor have the processes that control them. Using amino acid analysis, mass spectrometry and electron microscopy, we found that the lysyl hydroxylase inhibitor minoxidil, the matrix metalloproteinase inhibitor doxycycline, the PI3K/Akt pathway inhibitors wortmannin and the Akt1/2 inhibitor and drugs that affect the actin cytoskeleton significantly and selectively block the release of hydroxylysine and partially or completely suppress spreading of neutrophils. The actin cytoskeleton effectors and the Akt 1/2 inhibitor also increase the phenylalanine release. We hypothesize that hydroxylysine release upon adhesion is the result of the activation of lysyl hydroxylase in interaction with matrix metalloproteinase, the PI3K/Akt pathway and intact actin cytoskeleton, which play important roles in the recruitment of neutrophils into tissue through extracellular matrix remodeling.
- Subjects
AMINO acid analysis; AMINO acids; MATRIX metalloproteinase inhibitors; CYTOSKELETON; EXTRACELLULAR matrix; MASS spectrometry
- Publication
Cells (2073-4409), 2021, Vol 10, Issue 3, p563
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells10030563