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- Title
Evaluation of Immunogenicity and Protective Efficacy Elicited by Mycobacterium bovis BCG Overexpressing Ag85A Protein against Mycobacterium tuberculosis Aerosol Infection.
- Authors
Zheng Zhong Xu; Xiang Chen; Ting Hu; Chuang Meng; Xiao Bo Wang; Yan Rao; Xiao Ming Zhang; Yue Lan Yin; Zhi Ming Pan; Xin An Jiao
- Abstract
Mycobacterium bovis bacillus Calmette-Guérin (BCG) is currently the only vaccine available for preventing tuberculosis (TB), however, BCG has varying success in preventing pulmonary TB. In this study, a recombinant BCG (rBCG::Ag85A) strain overexpressing the immunodominant Ag85A antigen was constructed, and its immunogenicity and protective efficacy were evaluated. Our results indicated that the Ag85A protein was successfully overexpressed in rBCG::Ag85A, and the Ag85A peptide–MHC complexes on draining lymph node dendritic cells of C57BL/6 mice infected with rBCG::Ag85A were detectable 4 h post-infection. The C57BL/6 mice infected with this strain had stronger antigen-specific interferon-gamma (IFN-γ) responses and higher antibody titers than those immunized with BCG, and the protective experiments showed that rBCG::Ag85A can enhance protection against Mycobacterium tuberculosis (M. tuberculosis) H37Rv infection compared to the BCG vaccine alone. Our results demonstrate the potential of rBCG::Ag85A as a candidate vaccine against TB.
- Subjects
MYCOBACTERIUM bovis; DRUG efficacy; TUBERCULOSIS treatment; DENDRITIC cells; ANTIGENS
- Publication
Frontiers in Cellular & Infection Microbiology, 2016, Vol 6, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2016.00003