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- Title
Sensory innervation and inflammatory cytokines in hypertrophic synovia associated with pain transmission in osteoarthritis of the hip: a case–control study.
- Authors
Takeshita, Munenori; Nakamura, Junichi; Ohtori, Seiji; Inoue, Gen; Orita, Sumihisa; Miyagi, Masayuki; Ishikawa, Tetsuhiro; Takahashi, Kazuhisa
- Abstract
Objective. To clarify the sensory innervation and inflammatory cytokines in hypertrophic synovia associated with pain transmission in OA of the hip.Methods. A piece of the synovium was extracted during reconstruction surgery in 50 patients with OA of the hip as an inflammatory synovium and in 12 patients with femoral neck fracture as a normal synovium. Each sample was immersed in fixative solution, sectioned on a cryostat, and then processed for immunohistochemistry using antibodies as follows: neuron-specific class III β-tubulin (TuJ-1) as a general marker for nerve fibres, calcitonin gene-related peptide (CGRP) for sensory nerve fibres, nuclear factor κB (NF-κB) for the protein complex controlling the transcription of DNA in cellular responses to painful stimuli, and TNF-α for cytokines involved in acute inflammation. The number of immunopositive cells and fibres were counted using a fluorescence microscope.Results. In the inflammatory synovium of OA of the hip, TuJ-1 was positive in 46% (23 hips). Of those positive for TuJ-1, 78% (18 hips) were also positive for CGRP, but 22% (5 hips) were negative for CGRP. NF-κB was positive in 68% (34 hips). Of those positive for NF-κB, 76% (26 hips) were also positive for TNF-α, but 24% (8 hips) were negative for TNF-α. In normal synovia, all four substances were negative.Conclusion. We suggest sensory innervation and inflammatory cytokines in hypertrophic synovia are associated with nociception in OA of the hip.Trial registration: University Hospital Medical Information Network, www.umin.ac.jp, UMIN000001335.
- Subjects
JAPAN; ACADEMIC medical centers; CYTOKINES; FISHER exact test; HIP joint diseases; IMMUNOHISTOCHEMISTRY; LONGITUDINAL method; OSTEOARTHRITIS; PAIN; RESEARCH funding; U-statistics; SYNOVITIS; EQUIPMENT &; supplies; VISUAL analog scale; CASE-control method; DISEASE complications
- Publication
Rheumatology, 2012, Vol 51, Issue 10, p1790
- ISSN
1462-0324
- Publication type
Article
- DOI
10.1093/rheumatology/kes173