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- Title
Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus.
- Authors
Chengbao Wang; Han Meng; Yujin Gao; Hui Gao; Kangkang Guo; Almazan, Fernando; Sola, Isabel; Enjuanes, Luis; Yanming Zhang; Abrahamyan, Levon
- Abstract
In order to gain insight into the role of the transcription regulatory sequences (TRSs) in the regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus (PRRSV), the enhanced green fluorescent protein (EGFP) gene, under the control of the different structural gene TRSs, was inserted between the N gene and 3'-UTR of the PRRSV genome and EGFP expression was analyzed for each TRS. TRSs of all the studied structural genes of PRRSV positively modulated EGFP expression at different levels. Among the TRSs analyzed, those of GP2, GP5, M, and N genes highly enhanced EGFP expression without altering replication of PRRSV. These data indicated that structural gene TRSs could be an extremely useful tool for foreign gene expression using PRRSV as a vector.
- Subjects
PORCINE reproductive &; respiratory syndrome; GENETIC regulation; VIRAL replication; GREEN fluorescent protein; STRUCTURAL genomics
- Publication
Veterinary Research, 2017, Vol 48, p1
- ISSN
0928-4249
- Publication type
Article
- DOI
10.1186/s13567-017-0445-2