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- Title
Ketogenic Diet Has Moderate Effects on the Fecal Microbiota of Wild-Type Mice.
- Authors
Rohwer, Nadine; El Hage, Racha; Smyl, Christopher; Ocvirk, Soeren; Goris, Tobias; Grune, Tilman; Swidsinski, Alexander; Weylandt, Karsten-H.
- Abstract
The ketogenic diet (KD) is a high-fat, low-carbohydrate diet that has been reported to have neuroprotective effects. The health effects of KD might be linked to an altered gut microbiome, which plays a major role in host health, leading to neuroprotective effects via the gut-brain axis. However, results from different studies, most often based on the 16S rRNA gene and metagenome sequencing, have been inconsistent. In this study, we assessed the effect of a 4-week KD compared to a western diet (WD) on the colonic microbiome of female C57Bl/6J mice by analyzing fecal samples using fluorescence in situ hybridization. Our results showed distinct changes in the total number of gut bacteria following the 4-week KD, in addition to changes in the composition of the microbiome. KD-fed mice showed higher absolute numbers of Actinobacteria (especially Bifidobacteria spp.) and lower absolute levels of Proteobacteria, often linked to gut inflammation, in comparison with WD-fed mice. Furthermore, an increased abundance of the typically rare genus Atopobium was observed. These changes may indicate the possible anti-inflammatory effects of the KD. However, since the overall changes in the microbiota seem low, the KD effects might be linked to the differential abundance of only a few key genera in mice.
- Subjects
FECAL analysis; KETOGENIC diet; WESTERN diet; BIOLOGICAL models; GUT microbiome; ANIMAL experimentation; ANTI-inflammatory agents; ACTINOBACTERIA; COMPARATIVE studies; FLUORESCENCE in situ hybridization; RESEARCH funding; BACTERIA; MICE
- Publication
Nutrients, 2023, Vol 15, Issue 21, p4629
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15214629