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- Title
Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites.
- Authors
Todorova, Velislava; Savova, Martina S.; Ivanova, Stanislava; Ivanov, Kalin; Georgiev, Milen I.
- Abstract
Besides their common use as an adaptogen, Rhaponticum carthamoides (Willd.) Iljin. rhizome and its root extract (RCE) are also reported to beneficially affect lipid metabolism. The main characteristic secondary metabolites of RCE are phytoecdysteroids. In order to determine an RCE's phytoecdysteroid profile, a novel, sensitive, and robust high-performance thin-layer chromatography (HPTLC) method was developed and validated. Moreover, a comparative analysis was conducted to investigate the effects of RCE and its secondary metabolites on adipogenesis and adipolysis. The evaluation of the anti-adipogenic and lipolytic effects was performed using human Simpson–Golabi–Behmel syndrome cells, where lipid staining and measurement of released glycerol and free fatty acids were employed. The HPTLC method confirmed the presence of 20-hydroxyecdysone (20E), ponasterone A (PA), and turkesterone (TU) in RCE. The observed results revealed that RCE, 20E, and TU significantly reduced lipid accumulation in human adipocytes, demonstrating their anti-adipogenic activity. Moreover, RCE and 20E were found to effectively stimulate basal lipolysis. However, no significant effects were observed with PA and TU applications. Based on our findings, RCE and 20E affect both lipogenesis and lipolysis, while TU only restrains adipogenesis. These results are fundamental for further investigations.
- Subjects
PREVENTION of obesity; OBESITY; X-linked genetic disorders; STAINS &; staining (Microscopy); CELL physiology; RHUBARB; COMPARATIVE studies; TREATMENT effectiveness; PLANT extracts; CHROMATOGRAPHIC analysis; GLYCERIN; METABOLITES; LIPIDS; FATTY acids
- Publication
Nutrients, 2023, Vol 15, Issue 13, p3061
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15133061