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- Title
DNA-CHIP FOR DETERMINATION OF POINT MUTATIONS.
- Authors
Kökbaş, Umut; Şemsi, Rabia; Dincel, Aylin Sepici; Ergünol, Erdal; Tuli, Abdullah; Kayrın, Levent
- Abstract
Objective: Molecular diagnostics lies at the milestones of modern-day biochemistry. The identification of genetic and proteomic targets and patterns relies on the proper choice of detection methods, a combination of biochemical recognition architecture and signal processing. DNA-chip technology, by placing multiple probes on a single flats surface, allows for the storage of a tremendous amount of information and massively parallel detection of target species. The aim of this study, developing a new fast DNA-chip procedure for the detection of point mutations. Materials-Method: For this study, quartz crystal (qcm) electrodes surface modified with Poly Hema-Mac nanopolymer than, DNA-probes performed schiff base with the nanopolymer. Samples of genomic DNA were extracted from venous blood leukocytes and than, Arms (Amplification-Refractory Mutation System) method was used for creating amplicons. The amplicons were detected by using qcm DNAchip. We compare the results with gel electrophoresis. Results: The samples of wild type, α-thalassemia heterozygote, and homozygote samples PCR products were applied on the genosensor. When hybridization occurs on the electrode surface, quartz crystals frequency changes depend on the length of the probes. In this way, we can recognize the mutation type. Conclusıon: DNA-chip was evaluated for multiplex recognization of α- thalassemia mutations. This nanopolymer based piezoelectric DNAchip can be using an alternative technique for the determination of α- thalassemia mutations because it has more advantageous. For example, when this genosensor compared with conventional methods, it is faster, cheaper, more specific and less hazardous exposure.
- Subjects
DNA probes; QUARTZ crystals; GEL electrophoresis; MOLECULAR diagnosis; POINT mutation (Biology); ALPHA-Thalassemia; NANOSTRUCTURED materials
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2019, Vol 44, p15
- ISSN
0250-4685
- Publication type
Article