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- Title
Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay.
- Authors
Sierra-Arguello, Yuli Melisa; Quedi Furian, Thales; Perdoncini, Gustavo; Moraes, Hamilton L. S.; Salle, Carlos T. P.; Rodrigues, Laura B.; Ruschel dos Santos, Luciana; Pereira Gomes, Marcos José; Pinheiro do Nascimento, Vladimir
- Abstract
The objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one Campylobacter jejuni isolates (30 of poultry origin and 11 of human origin) and 11 Campylobacter coli isolates (10 of human origin and 1 of poultry origin) were examined for ciprofloxacin, norfloxacin, and nalidixic acid resistance using the minimal inhibitory concentration (MIC) method. Thereafter, the isolates were analyzed by PCR–Restriction Fragment Length Polymorphism (RFLP) assay for detection of Thr-86 mutation. Finally, DNA sequencing was performed for confirmation of gyrA gene mutation. A complete correlation was observed between MICs, PCR-RFLP assay, and sequencing. The results revealed high quinolone resistance rates for C. jejuni (100%) and C. coli (100%) isolates obtained from poultry and moderate resistance for C. jejuni (9.1%) and C. coli (40%) samples of human origin. A mutation in codon 86 of the gyrA gene with a Thr-to-Ile substitution is reported to be the main cause of high resistance to quinolones. This mutation can be analyzed by PCR-RFLP assay, which has been proven to be a simple and fast method for the detection of fluoroquinolone resistance in Campylobacter spp.
- Subjects
FLUOROQUINOLONES; DRUG resistance in bacteria; CAMPYLOBACTER jejuni; CAMPYLOBACTER coli; POLYMERASE chain reaction; RESTRICTION fragment length polymorphisms
- Publication
PLoS ONE, 2018, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0199974