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- Title
Engineering residues on C interface to improve thermostability of nitrilase for biosynthesis of Pregabalin precursor.
- Authors
Lu, Xia‐Feng; Tang, Xiao‐Ling; Xu, Chen‐Hui; Diao, Hong‐Juan; Wu, Zhe‐Ming; Zheng, Ren‐Chao; Zheng, Yu‐Guo
- Abstract
Nitrilase‐mediated bioprocesses exhibited great potential in the production of value‐added carboxylic acids. However, poor thermostability of nitrilases usually restricts their industrial applications. Herein, the thermostability of nitrilase BaNITM0 was significantly improved by engineering the amino acid residues on the intersection of two dimers (C interface). Except for simultaneous enhancement of enantioselectivity and activity, the best variant V82L/M127I/L159M/F166Q/C237S/Q260H (BaNITM4) showed a 10.8‐fold increase in half‐life at 30°C compared with BaNITM0. Structural analysis demonstrated that additional hydrogen bonds were formed between the residues on the C interface, which strengthened the interactions of two symmetrical regions and spirals. Subsequently, the engineered nitrilase was immobilized onto epoxy resins LXTE‐603 and the immobilized nitrilase exhibited excellent stability over 12 repeated cycles, which indicated a great industrial potential for biosynthesis of Pregabalin precursor.
- Subjects
EPOXY resins; AMINO acid residues; PREGABALIN; INDUSTRIAL capacity; BIOSYNTHESIS; CARBOXYLIC acids; HYDROGEN bonding
- Publication
AIChE Journal, 2023, Vol 69, Issue 11, p1
- ISSN
0001-1541
- Publication type
Article
- DOI
10.1002/aic.18211