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- Title
MicroRNA-223 regulates FOXO1 expression and cell proliferation
- Authors
Wu, Lihui; Li, Huihui; Jia, Cheng You; Cheng, Wei; Yu, Mei; Peng, Min; Zhu, Yuanqing; Zhao, Qianlei; Dong, Yi Wei; Shao, Kang; Wu, Anle; Wu, Xing Zhong
- Abstract
Abstract: In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-233 was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phosphorylation levels became significantly lower than those of their control. FOXO1 was down-regulated mainly in the cytoplasm, while the nuclear FOXO1 level became relatively high compared to the cytoplasm. As the unphosphorylated active form of FOXO1 increased in the cells, cyclin D1/p21/p27 were up-regulated at either mRNA or protein level. Proliferation of the cells was also greatly inhibited when miR-223 was over-expressed. Therein, our data suggest that miR-223 regulates FOXO1 expression and cell proliferation.
- Subjects
MICRORNA; GENE expression; CELL proliferation; COLON cancer; LENTIVIRUSES; PHOSPHORYLATION
- Publication
FEBS Letters, 2012, Vol 586, Issue 7, p1038
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.02.050