We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Antifatigue Activity of Glycoprotein from Schisandra chinensis Functions by Reducing Oxidative Stress.
- Authors
Shao, Shuai; Wang, Ming-xing; Zhang, Hong-yin; Fan, Lin; Han, Rong-xin; Shen, Ying-xin; Yan, Ming-ming; Zhao, Da-qing
- Abstract
The glycoprotein from Schisandra chinensis was obtained with alkali extraction and acid precipitation, purified with DEAE Sepharose Fast Flow and Superdex G-75 column. The molecular composition structure and antifatigue activities of glycoprotein were studied. SCGP's molecular weight was approximately 10 KDa, and it consisted of a carbohydrate component (52.94%) and protein component (47.06%). SCGP comprised mannose, galactoside, rhamnose, glucose, galactose, xylose, arabinose, and fucose, its molar ratio was 2.14 : 1.43 : 1.59 : 8.17 : 8.99 : 3.18 : 18.51 : 1, and it contained 16 kinds of amino acids. SCGP could obviously extend the swimming time in mice by increasing LDH, SOD level, GSH-Px activity, and liver glycogen and decreasing the contents of BUN and MDA. The antioxidant activity of SCGP is a potential mechanism of its antifatigue effect. In vitro antioxidant test showed that SCGP scavenged DPPH and OH radicals in a dose-dependent manner (IC50 was 0.91 mg/ml and 0.72 mg/ml).
- Subjects
AMINO acid analysis; GLUCOSE analysis; ANIMAL experimentation; DEOXY sugars; DOSE-effect relationship in pharmacology; FATIGUE (Physiology); FRUIT; GLYCOGEN; GLYCOPROTEINS; GLYCOSIDES; HERBAL medicine; LACTATE dehydrogenase; LIVER; CHINESE medicine; MICE; MONOSACCHARIDES; SUPEROXIDE dismutase; SWIMMING; MALONDIALDEHYDE; OXIDATIVE stress; HEXOSES; GLUTATHIONE peroxidase; BLOOD urea nitrogen; IN vitro studies
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2020, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2020/4231340