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- Title
Effect of Moxibustion on Intestinal Microbiome in Acute Gastric Ulcer Rats.
- Authors
He, Qi-da; Huang, Miao-sen; Zhang, Long-bin; Shen, Jia-cheng; Lian, Lin-yu; Zhang, Yuan; Chen, Bao-hua; Liu, Cai-chun; Qian, Lin-chao; Liu, Mi; Yang, Zong-bao
- Abstract
In Traditional Chinese Medicine (TCM), moxibustion had been used for thousands of years. Many clinical case reports and scientific studies had proved that moxibustion had a good effect in treating acute gastric ulcer (AGU). Some studies had shown that the relative content and species of bacteria in the intestinal would be changed when gastric mucosal injury happened. However, there was little research on the effect of intestinal microbiome with AGU rats that were treating by moxibustion. This study is aimed at analyzing the effect of fecal microbiome in rats with AGU by the 16S rDNA sequencing technology. Male SD rats were established by orally feeding once with 70% ethanol at 4 ml/kg except the control group, then treated by moxibustion in the stomach meridian group ("Liangmen," "Zusanli") and the gallbladder meridian group ("Riyue," "Yanglingquan") for 5 days. The 16S rDNA sequencing technology analysis of feces combined with histopathological methods and molecular biological detection methods was used to evaluate the therapeutic mechanism of moxibustion on AGU. AGU brought cause changes in the number and species of intestinal bacteria. Moxibustion on stomach meridian group could reduce the area of gastric mucosal injury and regulate the relative content of GAS and EGF. Moreover, moxibustion on the stomach meridian group could increase the relative content and species of beneficial bacteria in the intestine of rats with AGU. The relative abundance of intestinal probiotics was significantly upregulated in Alphaproteobacteria, Actinomycetales, and Bacillales. In addition, moxibustion might promote the repair of gastric mucosal injury by increasing the number and species of beneficial bacteria in the intestine.
- Subjects
ACTINOMYCETALES; ANIMAL experimentation; BACILLACEAE; DNA; ETHANOL; FECES; GALLBLADDER; HISTOLOGICAL techniques; MOLECULAR biology; MOXIBUSTION; ORAL drug administration; PEPTIC ulcer; RATS; STOMACH; GUT microbiome; PROBIOTICS; TREATMENT effectiveness; ACUTE diseases; SEQUENCE analysis
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2019, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2019/6184205