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- Title
Definitive Structural Assessment of Enterococcal Diheteroglycan.
- Authors
Krylov, Vadim B.; Gerbst, Alexey G.; Argunov, Dmitry A.; Dmitrenok, Andrey S.; Shashkov, Alexander S.; Kaczynski, Zbigniew; Huebner, Johannes; Holst, Otto; Nifantiev, Nikolay E.
- Abstract
Enterococcus faecalis is one of most important nosocomial and often multi-antibiotic resistant pathogens responsible for infections that are difficult to treat. Previously, a cell-wall polysaccharide termed diheteroglycan (DHG) was isolated and characterized as a promising vaccine candidate. However, the configuration of its lactic acid (LA) residue attached to the galactofuranoside unit was not assessed, although it influences conformation of DHG chain in terms of biological recognition and immune evasion. This study proves the R configuration of the LA residue by means of chemical analysis, investigation of intramolecular NMR nuclear Overhauser effects and molecular dynamics simulations of native DHG and corresponding R and S models, which were obtained by using pyranoside- into-furanoside rearrangement. As alternative treatment and prevention strategies for E. faecalis are desperately needed, this discovery may offer the prospect of a synthetic vaccine to actively immunize patients at risk.
- Subjects
ENTEROCOCCUS faecalis; BACTERIAL cell walls; OVERHAUSER effect (Nuclear physics); MOLECULAR dynamics; FURANOSIDES
- Publication
Chemistry - A European Journal, 2015, Vol 21, Issue 4, p1749
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201405857