We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Rapid and simultaneous measurement of phosphorus metabolite pool size ratio and reaction kinetics of enzymes in vivo.
- Authors
Kim, Sang‐Young; Chen, Wei; Ongur, Dost; Du, Fei; Kim, Sang-Young
- Abstract
<bold>Purpose: </bold>The metabolites phosphocreatine (PCr), adenosine triphosphate (ATP), and in-organic phosphate (Pi) are biochemically coupled. Their pool sizes, assessed by their magnetization ratios, have been extensively studied and reflect bioenergetics status in vivo. However, most such studies have ignored chemical exchange and T1 relaxation effects. In this work, we aimed to extend the T1nom method to simultaneously quantify the reaction rate constants as well as phosphorus metabolite pool size ratios under partially relaxed conditions.<bold>Materials and Methods: </bold>Modified Bloch-McConnell equations were used to simulate the effects of chemical exchanges on T1 relaxation times and magnetization ratios among PCr, γ-ATP, and Pi. The T1nom method with iteration approach was used to measure both reaction constants and metabolite pool size ratios. To validate our method, in vivo data from rat brains (N = 8) at 9.4 Tesla were acquired under two conditions, i.e., approximately full relaxation (TR = 9 s) and partial relaxation (TR = 3 s). We compared metabolite pool size ratios and reaction constants before and after correcting the chemical exchange and T1 relaxation effects.<bold>Results: </bold>There were significant errors in underestimation of PCr/γATP by 12 % (P = 0.03) and overestimation of ATP/Pi ratios by 14 % (P = 0.04) when not considering chemical exchange effects. These errors were minimized using our iteration approach, resulting in no significant differences (PCr/γATP, P = 0.47; ATP/Pi, P = 0.81) in metabolite pool size ratios and reaction constants between the two measurements (i.e., short versus long TR conditions).<bold>Conclusion: </bold>Our method can facilitate broad biomedical applications of 31 P magnetization saturation transfer spectroscopy, requiring high temporal and/or spatial resolution for assessment of altered bioenergetics.<bold>Level Of Evidence: </bold>2 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2018;47:210-221.
- Subjects
ADENOSINE triphosphate; ALGORITHMS; ANIMAL experimentation; BRAIN; COMPUTER simulation; DYNAMICS; ENERGY metabolism; MAGNETIC resonance imaging; NUCLEAR magnetic resonance spectroscopy; PHOSPHATES; PHOSPHOCREATINE; PHOSPHORUS; RATS; RESEARCH evaluation; RESEARCH funding; STATISTICAL models
- Publication
Journal of Magnetic Resonance Imaging, 2018, Vol 47, Issue 1, p210
- ISSN
1053-1807
- Publication type
journal article
- DOI
10.1002/jmri.25744