We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Development of a Method for the Quantification of Clotrimazole and Itraconazole and Study of Their Stability in a New Microemulsion for the Treatment of Sporotrichosis.
- Authors
Garcia Ferreira, Patricia; Guimarães de Souza Lima, Carolina; Noronha, Letícia Lorena; de Moraes, Marcela Cristina; Silva, Fernando de Carvalho da; Lifsitch Viçosa, Alessandra; Omena Futuro, Débora; Francisco Ferreira, Vitor; Locatelli, Clinio; Locatelli, Marcello; Melucci, Dora
- Abstract
Sporotrichosis occurs worldwide and is caused by the fungus Sporothrix brasiliensis. This agent has a high zoonotic potential and is transmitted mainly by bites and scratches from infected felines. A new association between the drugs clotrimazole and itraconazole is shown to be effective against S. brasiliensis yeasts. This association was formulated as a microemulsion containing benzyl alcohol as oil, Tween® 60 and propylene glycol as surfactant and cosurfactant, respectively, and water. Initially, the compatibility between clotrimazole and itraconazole was studied using differential scanning calorimetry (DSC), thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), and X-ray powder diffraction (PXRD). Additionally, a simple and efficient analytical HPLC method was developed to simultaneously determine the concentration of clotrimazole and itraconazole in the novel microemulsion. The developed method proved to be efficient, robust, and reproducible for both components of the microemulsion. We also performed an accelerated stability study of this formulation, and the developed analytical method was applied to monitor the content of active ingredients. Interestingly, these investigations led to the detection of a known clotrimazole degradation product whose structure was confirmed using NMR and HRMS, as well as a possible interaction between itraconazole and benzyl alcohol.
- Subjects
MICROEMULSIONS; ITRACONAZOLE; CLOTRIMAZOLE; FOURIER transform infrared spectroscopy; BENZYL alcohol; X-ray powder diffraction; THERAPEUTICS
- Publication
Molecules, 2019, Vol 24, Issue 12, p2333
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules24122333