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- Title
Identification of Anti-Melanogenesis Constituents from Morus alba L. Leaves.
- Authors
Li, Hong Xu; Park, Jung Up; Su, Xiang Dong; Kim, Kyung Tae; Kang, Jong Seong; Kim, Young Ran; Kim, Young Ho; Yang, Seo Young; Ferreira, Isabel C. F. R.
- Abstract
The individual parts of Morus alba L. including root bark, branches, leaves, and fruits are used as a cosmetic ingredient in many Asian countries. This study identified several anti-melanogenesis constituents in a 70% ethanol extract of M. alba leaves. The ethyl acetate fraction of the initial ethanol extract decreased the activity of tyrosinase, a key enzyme in the synthetic pathway of melanin. Twelve compounds were isolated from this fraction and their structures were identified based on spectroscopic spectra. Then, the authors investigated the anti-melanogenesis effects of the isolated compounds in B16-F10 mouse melanoma cells. Compounds 3 and 8 significantly inhibited not only melanin production but also intracellular tyrosinase activity in alpha-melanocyte-stimulating-hormone (α-MSH)-induced B16-F10 cells in a dose-dependent manner. These same compounds also inhibited melanogenesis-related protein expression such as microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein-1 (TRP-1). Compound 3 modulated the cAMP-responsive element-binding protein (CREB) and p38 signaling pathways in α-MSH-activated B16-F10 melanoma cells, which resulted in the anti-melanogenesis effects. These results suggest that compound 3, isolated from M. alba leaves, could be used to inhibit melanin production via the regulation of melanogenesis-related protein expression.
- Subjects
WHITE mulberry; EXTRACTS -- Therapeutic use; ETHYL acetate; MELANOGENESIS; MELANINS; CARRIER proteins; PROTEIN expression
- Publication
Molecules, 2018, Vol 23, Issue 10, p2559
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules23102559