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- Title
Metabolic Profiling of Lactococcus lactis Under Different Culture Conditions.
- Authors
Azizan, Kamalrul Azlan; Baharum, Syarul Nataqain; Noor, Normah Mohd
- Abstract
Gas chromatography mass spectrometry (GC-MS) and headspace gas chromatography mass spectrometry (HS/GC-MS) were used to study metabolites produced by Lactococcus lactis subsp. cremoris MG1363 grown at a temperature of 30 °C with and without agitation at 150 rpm, and at 37 °C without agitation. It was observed that L. lactis produced more organic acids under agitation. Primary alcohols, aldehydes, ketones and polyols were identified as the corresponding trimethylsilyl (TMS) derivatives, whereas amino acids and organic acids, including fatty acids, were detected through methyl chloroformate derivatization. HS analysis indicated that branched-chain methyl aldehydes, including 2-methylbutanal, 3-methylbutanal, and 2-methylpropanal are degdradation products of isoleucine, leucine or valine. Multivariate analysis (MVA) using partial least squares discriminant analysis (PLS-DA) revealed the major differences between treatments were due to changes of amino acids and fermentation products.
- Subjects
LACTOCOCCUS lactis; GAS chromatography/Mass spectrometry (GC-MS); ORGANIC acids; TRIMETHYLSILYL compounds; MULTIVARIATE analysis; AMINO acids
- Publication
Molecules, 2012, Vol 17, Issue 7, p8022
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules17078022