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- Title
Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnostics.
- Authors
Domnich, Alexander; De Pace, Vanessa; Pennati, Beatrice M.; Caligiuri, Patrizia; Varesano, Serena; Bruzzone, Bianca; Orsi, Andrea
- Abstract
Extraction-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is currently the "gold standard" in SARS-CoV-2 diagnostics. However, some extraction-free RT-qPCR techniques have recently been developed. In this study, we compared the sensitivity of traditional extraction-based, heated extraction-free, and unheated extraction-free RT-qPCR methods for SARS-CoV-2 detection in nasopharyngeal swabs from symptomatic individuals. The unheated extraction-free method showed perfect agreement with the standard extraction-based RT-qPCR. By contrast, the heat-treated technique was associated with an 8.2% false negativity rate. Unheated extraction-free RT-qPCR for the molecular diagnosis of SARS-CoV-2 is a valuable alternative to the traditional extraction-based methods and may accelerate turnaround times by about two hours.
- Subjects
SARS-CoV-2; REVERSE transcriptase polymerase chain reaction
- Publication
Archives of Virology, 2021, Vol 166, Issue 10, p2825
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-021-05165-0