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- Title
Infection of cynomolgus macaques with a recombinant monkeypox virus encoding green fluorescent protein.
- Authors
Goff, Arthur; Mucker, Eric; Raymond, Jolynne; Fisher, Robert; Bray, Mike; Hensley, Lisa; Paragas, Jason
- Abstract
Monkeypox virus (MPXV) causes a vesiculopustular rash illness resembling smallpox in humans and produces a similar disease in nonhuman primates. To enhance the ability of researchers to study experimental MPXV infections, we inserted a gene encoding green fluorescent protein (GFP) into Monkeypox virus Zaire-79. Wild-type and MPXV-GFP replicated with similar kinetics in cell culture and caused a similar disease when injected intravenously into cynomolgus macaques. In MPXV-GFP-infected animals, examination under fluorescent light facilitated the identification of skin lesions during disease development and internal sites of replication at necropsy. MPXV-GFP could improve the quantitative assessment of antiviral therapy and vaccine efficacy.
- Subjects
MACAQUES; MONKEYPOX virus; RECOMBINANT viruses; GREEN fluorescent protein; GENETIC code; CELL culture; FLUORESCENT lighting; VIRAL replication; DISEASES
- Publication
Archives of Virology, 2011, Vol 156, Issue 10, p1877
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-011-1065-1