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- Title
Effect of 3D Chondrocyte Culturing Conditions on the Formation of Extracellular Matrix in Cartilage Tissue-Engineering Constructs.
- Authors
Ponomarev, I.; Kochneva, L.; Barnewitz, D.
- Abstract
We studied biochemical, morphological, and histological parameters of the extracellular matrix in scaffold-free tissue engineering chondrotransplants prepared from chondrocytes isolated from knee joint cartilage biopsy specimens of Haflinger horses (age 3.5-14 years) and in transplants prepared on the basis of commercial matrixes of Ethisorbff and Chondro-Gideff. A total of 50 tissue-engineering constructs were designed and analyzed. Passage 2 cells populations were used. Mechanical stimulation during culturing of scaffold-free constructs considerably activated synthesis of the basic components of the cartilage matrix (proteoglycan concentration was 35% of the content in the native tissue, and the content of collagen-specific amino acids hydroxyproline and hydroxylysine attained 29.3 and 12.7%, respectively). The architecture of these cartilage constructs was morphologically and histologically similar to the native cartilage tissue. Insufficient support of the chondrogenesis by scaffold-based chondrotransplants and no differences between these constructs by the studied parameters were noted despite different chemical nature and structure of these scaffolds.
- Subjects
CARTILAGE cell transplantation; EXTRACELLULAR matrix; CHONDROGENESIS; CELL culture; BIOPSY; TISSUE engineering; HAFLINGER horse; PROTEOGLYCANS; HYDROXYPROLINE
- Publication
Bulletin of Experimental Biology & Medicine, 2014, Vol 156, Issue 4, p548
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-014-2394-3